Long noncoding RNA H19 promotes leukocyte inflammation in ischaemic
stroke by targeting the miR-29b/C1QTNF6 axis
Abstract
Background and Purpose Inflammatory processes induced by leukocytes are
crucially involved in the pathophysiology of acute ischemic stroke. This
study aimed to elucidate the inflammatory mechanism of long non-coding
RNA (lncRNA) H19-mediated regulation of C1QTNF6 by sponging miR-29b in
leukocytes during ischemic stroke. Experimental Approach H19 and miR-29b
expression in leukocytes of patients with ischemic stroke and middle
cerebral artery occlusion rats was measured through real-time PCR.
Moreover, we performed in vivo and in vitro experiments to determine the
impact of H19 and miR-29b on C1QTNF6 expression in leukocytes after
ischemic injury. Key Results There was H19 and C1QTNF6 upregulation, as
well as miR-29b downregulation, in neutrophils of patients with stroke.
Moreover, miR-29b could bind C1QTNF6 mRNA to repress its expression
while H19 could sponge miR-29b to maintain C1QTNF6 expression. Moreover,
C1QTNF6 overexpression promoted the release of IL-1β and TNF-α in
leukocytes and further exacerbates blood-brain barrier disruption.
Conclusion and Implications Our findings confirm the
inflammation-modulatory effect of the H19/miR-29b/C1QTNF6 axis on
cerebral ischemic injury.