Abstract
Background: Random-pattern skin flaps are often utilized to cover
numerous skin defects that can occur for a variety of causes, although
tissue ischemia is the most prevalent consequence that leads to surgery
failure. Protein Kinase D1 (PKD1), a calcium/calmodulin-dependent
serine/threonine kinase, has been linked to angiogenesis and has been
shown to protect against ischemic cardiovascular disease in several
studies. However, no relevant studies on skin flaps have been recorded.
Methods: Sixty male Sprague-Dawley rats were randomly separated into
control, PKD1, and CID755673 group. We observed postoperative survival,
laser Doppler and lead oxide/gelatin angiography were used to assess
blood flow, HE (hematoxylin and eosin) staining was used to observe
neovascularization, and determined the level of related protein
expression through Immunoblotting and immunohistochemistry. Results: The
PKD1 group has the largest survival area and the most abundant blood
supply. The level of angiogenesis and oxidative stress is the highest,
and the level of apoptosis is the lowest. The CID755673 group is the
opposite. Conclusion: The findings show that PKD1 increases the flap’s
survival rate and is linked to increased angiogenesis, reduced oxidative
stress, and apoptosis inhibition.