Dynamic light scattering (DLS)
IgG (0.5–1.0 mg/mL), treated and untreated, was solubilized in 50 mM
glycine and 30 mM NaCl at pH 3.8. Samples were centrifuged at 13K rpm
for 20 minutes and the supernatant collected for analysis. The
intensity-weighted size distributions of human and mouse IgG samples
were determined using the auto correlation spectroscopy protocol of the
Nanophox instrument (Sympatec GmbH, Germany).