Preparation of detergent aggregates
Detergent aggregates were obtained by mixing equal volumes of medium A and B as follows: medium A was prepared by the addition of 20 μL of the hydrophobic chelator bathophenanthroline (20 mM in methanol) to 180 μL of: 0.25 mM; 0.215 mM; 0.062 mM; 0.062 mM; 0.1 mM; 0.09 mM; 0.05mM; 0.1 mM and 0.5 mg/mL of Tween-20, Tween-40, Tween-60, Tween-80, Brij C-10, Brij O-20, Brij S-100, Triton X-100 and Pluronic F-127 in DDW, respectively, with vigorous vortexing to a final volume of 200 μL. An equal volume of medium B , containing 1 mM FeSO4 in 20 mM NaCl was then added to medium A with vigorous vortexing.