Figure 2: A. SDS-PAGE analysis of captured
IgG’s in pellets containing non-ionic detergents. A . Pellet
composition: Lane 1: Control: total hIgG and BSA; Lanes 2-5: Pellet
composition after hIgG capture in the presence of the Tween
representatives; Lanes 6-8: as in lanes 2-5 but in the presence of Brij
representatives; Lane 9 and 10 as in lanes 2-5 but in the presence of
Triton X-100 and Pluronic F-127, respectively. B . As inA , but with mouse IgG. BSA , H , L,A denote bovine serum albumin, the reduced h eavy
chain, the reduced l ight chain and the detergenta ggregates, respectively. C-D . Supernatant composition
after extracting IgG’s from indicated
[detergent:[(batho)3:Fe2+]
pellets with 50 mM glycine at pH 3.8 as described in Experimental.
Indicated overall process yields (i.e. IgG capture and
extraction) are shown and represent an average of 3 independent
experiments. Gels are Coomassie stained.