Extraction of IgG’s from detergent aggregates
Detergent aggregates containing the target IgG were incubated with 100
μL of: 50 mM Gly, 30 mM NaCl at pH 3.8 for 5 minutes at room
temperature. Centrifugation followed (13K rpm, 2 min.) and the
supernatant was carefully removed for further analysis by pipetation or
filtered using PVDF filters with a pore size of 0.1 µm (Millipore -
Ultrafree ® MC- VV).