Comparison with Protein A spin columns
The IgG:BSA sample, prepared as described above, was applied to commercial Protein A spin column and purification was performed according to manufacturer instructions (General Electric). Eluted IgG’s were then compared to those purified using Tween-20, Tween-40, Tween-60, Tween-80, Brij C-10, Brij O-20, Brij S-100, Triton X-100 and Pluronic F-127 aggregates.