Figure 4: Purification of IgG’s via filtration.A. Characterization of detergent aggregates for purifying hIgG
under conditions described in the Experimental section, where
centrifugation is replaced by filtration. BSA , H ,L denote bovine serum albumin, the reduced h eavy
chain, the reduced l ight chain, respectively. The asterisks
represent overall yields based on 4-6 independent experiments. Gels are
Coomassie stained. B. Dynamic light scattering (DLS) analysis
of hIgG purified via filtration with detergents as indicated.
Control - hIgG particle size (10.95 nm), not subject to detergent
aggregate purification. C. ELISA analysis. Anti-BSA IgG was
captured and extracted from indicated detergent aggregates and tested
for its ability to recognize BSA via an ELISA assay described in the
Experimental. Indicated percent values represent IgG recovery yields
relative to equal amounts of IgG not exposed to detergent aggregates
(control). Filters used were made of PVDF with a pore size of 0.1 µm
(Millipore - Ultrafree ® MC- VV).