Dynamic light scattering (DLS)
IgG (0.5–1.0 mg/mL), treated and untreated, was solubilized in 50 mM glycine and 30 mM NaCl at pH 3.8. Samples were centrifuged at 13K rpm for 20 minutes and the supernatant collected for analysis. The intensity-weighted size distributions of human and mouse IgG samples were determined using the auto correlation spectroscopy protocol of the Nanophox instrument (Sympatec GmbH, Germany).