Purification of hIgG and mouse IgG with detergent aggregates
Freshly prepared aggregates were resuspended in 100 μL of serum-free
medium (Ex-CELL 610-HSF) containing: 4% PEG-6000, the target IgG (1
g/mL) and BSA (0.25 mg/mL). After 5 minutes of incubation at room
temperature, centrifugation (14K, 2 min.) was applied, the supernatant
discarded and pellets were briefly washed with 100 μL of cold 20 mM
NaCl. An additional centrifugation step followed (14K rpm, 2 min.), the
supernatant was removed, and pellets were analyzed by SDS-PAGE.