Extraction of IgG’s from detergent aggregates
Detergent aggregates containing the target IgG were incubated with 100 μL of: 50 mM Gly, 30 mM NaCl at pH 3.8 for 5 minutes at room temperature. Centrifugation followed (13K rpm, 2 min.) and the supernatant was carefully removed for further analysis by pipetation or filtered using PVDF filters with a pore size of 0.1 µm (Millipore - Ultrafree ® MC- VV).