Conclusions
Detergent aggregates composed of conjugated Tween-20, Brij-O20 or Triton
X-100 nonionic micelles and the amphiphilic
[(batho)3:Fe2+] red complex appear
to represent an efficient and economical platform for IgG purification.
The possibility of integrating this platform into industrial, continuous
production flows is supported by the findings that: (i)filtration can replace centrifugation and lead to highly pure, recovered
Abs (>95%) with improved overall recovery yields
(79-90%); (ii) process efficiency is not significantly reduced
at higher IgG concentrations (e.g. 3-5 mg/mL); and (iii)chelator recycling is almost quantitative (95%). (iv) The
process is rapid: on average, only 15-20 minutes are needed to capture
the target IgG and recover it in pure form via the filtration
protocol. This should translate into significant time saving and hence,
higher manufacturing productivity. However, the true practical and
economic value of the IgG purification platform for downstream
processing of therapeutic grade mAbs will now require additional process
upscaling.