Materials and methods
Materials: Ethylenediaminetetraacetic acid (Sigma, E6758), Bovine serum
albumin (Sigma, A7906), Protein A HP Spin-Trap (Sigma, 28903132), iron
(II) sulfate heptahydrate (Sigma, F7002), sodium chloride (Sigma,
S7653), polysorbate 20 (Sigma, 44112), polysorbate 40 (Sigma, P1504),
polysorbate 60 (Sigma, P1629), polysorbate 80 (Sigma, P1754), Brij C-10
(Sigma, 388858), Brij O-20 (Sigma, 436240), Brij S-100 (Sigma, 466387),
Triton X-100 (Sigma, laboratory grade), Pluronic F-127 (Sigma, P2443)
Ex-CELL 610-HSF medium (Sigma, 14610C), glycine (Bio-lab 07132391),
bathophenanthroline (GFS chemicals, C038446), streptavidin-HRP conjugate
(RD system, 321894), TMB solution (eBioscience,:00-4201), PBS buffer 10X
(Bio-Lab, 00162323G500), human IgG (Lee-Biosicences, 340-21), mouse IgG
(Equitech, SLM66).