Figure 4: Purification of IgG’s via filtration.A. Characterization of detergent aggregates for purifying hIgG under conditions described in the Experimental section, where centrifugation is replaced by filtration. BSA , H ,L denote bovine serum albumin, the reduced h eavy chain, the reduced l ight chain, respectively. The asterisks represent overall yields based on 4-6 independent experiments. Gels are Coomassie stained. B. Dynamic light scattering (DLS) analysis of hIgG purified via filtration with detergents as indicated. Control - hIgG particle size (10.95 nm), not subject to detergent aggregate purification. C. ELISA analysis. Anti-BSA IgG was captured and extracted from indicated detergent aggregates and tested for its ability to recognize BSA via an ELISA assay described in the Experimental. Indicated percent values represent IgG recovery yields relative to equal amounts of IgG not exposed to detergent aggregates (control). Filters used were made of PVDF with a pore size of 0.1 µm (Millipore - Ultrafree ® MC- VV).