In the methodology, there is no reference of what the authors consider the low levels of ethanol; moreover, the statement that 20 mM EtOH is equivalent to 0.04 dg/L BAC lacks a reference or appropriate demonstration in larvae.
The term "Pavlovian fear conditioning" is inadequate to describe the aversive conditioning task used; in fact, what the authors are performing is avoidance conditioning, which has initial components of Pavlovian conditioning (CS-US) followed by instrumental conditioning (CS-CR).
In the procedure described for the task of appetitive conditioning it is mentioned that after training the fish were exposed toof 5 trials of initiator light “on” (30-sec), or “off” (30-sec), however, in results is described that the fish were presented with the stimulus light 5-times (10-sec), interspersed with non-light presentations (10-sec).
It might be interesting to elaborate a percentage representation of figure 6A (referring to the frequency of choice in Y-maze test of perseveration and repetitive alternation ) in which thetagrams representing the mode of random exploration were grouped into a single group to facilitate comparison between the random mode of operation and the other modes (alternating and repetitive)