Growth inhibition effects of NCTD on cancer cell lines and
LO2 cells
To determine whether NCTD has a cytotoxic effect on human cancer cells,
we assessed the viability of human cancer cell lines (non-small lung
cancer cell line A549, breast cancer MCF-7 cell line , hepatocellular
carcinoma BEL-7402 cell line, nasopharyngeal carcinoma CNE-2 cell line)
and human hepatic cell line LO2. Cells were exposed to different
concentrations of NCTD for 72 h as revealed in Fig. 1B. NCTD
showed significant proliferation inhibition and cytotoxic effects on
these cancer cell lines, and showed moderate cytotoxic effects on normal
LO2 cells. These data indicate that NCTD strongly inhibits tumor cell
proliferation in a dose- and time-dependent manner. After 72 h of cell
exposure, NCTD inhibited A549 cell activity in a dose-dependent manner
with an IC50 value of 13.1 μM (Table 1). In addition, the clone
formation test showed that NCTD significantly inhibited the cloning
ability of A549 cells (Figure 1C).
fig. 1
table 1