Effect of NCTD on the expression of the Akt/mTOR pathway and the JNK/c-jun pathway
PI3K / Akt / mTOR, AMPK / mTOR and JNK play important regulatory functions in various cell life activities, such as apoptosis, cell cycle rhythm, differentiation, growth, etc. We examined the expression of p-Akt, p-AMPK, mTOR, p-JNK and p-c-jun in NCTD-affected A549 cells by Western blot. As shown in Figure 6A, the NCTD concentration and the expression of p-Akt and mTOR showed a negative correlation. However, it is positively correlated with p-AMPK and ULK1. In addition, the expression of p-JNK and its downstream protein pc-jun gradually increased with increasing NCTD concentration in treated cells (Figure 6B). The above data indicate that the activation and inhibition of the AMPK / mTOR and JNK / c-jun pathways are involved in the Akt / mTOR signal transduction pathway and are related to apoptosis and autophagy of A549 cells.
Fig6.
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