Inhibition
On average inhibition caused qPCR reactions to be delayed with 1.05
cycle. In 17% of the reactions performed the qPCR was delayed for more
than 2 cycles. We found that qPCR reactions with 33 template DNA
molecules were 3.3 times more often inhibited for more than 2 cycles
than qPCR reactions with 3333 template DNA molecules. However,
inhibition was not significantly stronger at low DNA concentration
(slope of regression did not differ from 1 T=-0.2081434, df=21,
p>0.4 ). The model that best supported the data did not
include pH or OM as factors. Therefore, we assumed that the low levels
of inhibition would affect all treatments
equally.