3.3 | Gene ontology enrichment
BLAST search resulted in only six loci being associated with known genes in 2014 and 24 in 2015 (Supplementary materials, Table 2). The broad-scale biological processes associated with the gene ontology (GO) enrichment are listed in (Supplementary materials, Table 3), while detailed information and fine-scale biological processes associated with the gene subsets may be found in the supplementary materials. The majority of genes were associated with developmental processes: 4 out 5 in 2014 and 108 out of 168 in 2015 (Supplementary materials, Table 3). Intracellular processes were associated with all environmental gradients in both years (see supplementary materials). Various developmental processes were mostly associated with collection date, chlorophyll-a concentration, latitude, water temperature, as well as tissue lipid percentage. Growth associated processes were mostly associated with chlorophyll-a concentration, collection date, collection latitude, water temperature and tissue lipid percent, but not condition index. Metabolic related processes were associated with condition index, latitude, and temperature. Fatty acid and lipid metabolism processes were only associated with a temperature gradient.