Tables and Figures
Table 1. Comparison of main genomic characteristics. Louse
symbionts highlighted by grey background.
Figure 1. Phylogenetic relationships of the two
Neisseria-related symbionts. Multigene matrix: Bayesian analysis of the
multigene matrix; the numbers at the nodes show posterior
probabilities/bootstrap supports obtained by the Maximum Likelihood
analysis in PhyML. 16S matrix: part of the tree obtained by the Bayesian
analysis of the 16S matrix showing relationships of the two
Neisseria-related symbionts to several Uncultured bacteria (see
SupplementaryInformation/SupplementaryFigure1 for the complete tree);
the numbers at the nodes show posterior probabilities/bootstrap supports
obtained by the Maximum Likelihood analysis in PhyML. Mauve synteny: an
overview of strong synteny between the two symbionts; H -
“Neisseria”-H, P - “Neisseria”-P (see SupplementaryData1 for a
complete list of the genes).
Figure 2. Highly abundant taxa found in H. edentula and four
different populations of H. acanthopus. Detailed phylogenetic
relationships of the hosts are shown in the
SupplementaryInformation/SupplementaryFigure2.
Figure 3. Highly abundant taxa found in Polyplax serrata
samples from distinct populations. The phylogenetic scheme simplifies
the COI based phylogeny of individual samples provided in
SupplementaryInformation/SupplementaryFigure3. Designation of the
branches is based on mtDNA structure described in the study by Martinu
et al. [50]: A = lineage specific to Apodemus agrarius, S = lineage
specific to Apodemus flavicollis (W = west sublineage, E = east
sublinage), N= nonspecific lineage from A. flavicollis and A.
sylvaticus. The numbers for Legionella polyplacis designate two
different OTUs, reflecting evolutionary changes accumulated after the
split of Polyplax serrata lineages.
Figure 4. Light and confocal microscopy of a FISH-stained
female H. acanthopus . (a) Light microscopy image showing the
louse body containing a developing egg (white arrow). (b) Hybridization
signal for the Neisseriaceae specific probe beta-572 (green) shows the
localization of the symbionts. The dashed red square defines the region
shown in panel c, d and e. (c, d and e) Hybridization signals for DAPI
(cyan), the Neisseriaceae specific probe beta-572 (green) and the
generic bacterial probe EUB338 (magenta) are shown in panel c, d and e,
respectively, and were combined in the merged color image (f), with theNeisseria ‑related symbionts appearing white. DAPI staining in
panel c defines also the localization of the bacteriocytes above ovarial
ampullae.
Scale bars: (a, b) 500 μm, (c, d, e and f) 20 μm.