Inoculation experiment and
qPCR
We detected powdery mildew spores growing on the inoculated, susceptible
plant clone leaves on day 14 post inoculation. None of the
mock-inoculated or inoculated resistant plant clones showed visible
disease symptoms at that time. Gene expression of the marker genes
varied considerably in the susceptible inoculated plants and showed
elevated levels only at time point 72 h post inoculation (Supplementary
Figure 1). This time point was chosen for further analysis.