Plant transformation
Constructs in binary vectors were introduced into Agrobacterium
tumefaciens strain LBA4404 and transformed into Arabidopsis WT
or mutant plants by the floral-dip
method(Clough & Bent, 1998).
Approximately 30 T1 transgenic plants for each transgene were screened
on MS medium supplemented with the appropriate antibiotics, and
phenotypic analyses were performed in T2 or later generations.