Plant transformation
Constructs in binary vectors were introduced into Agrobacterium tumefaciens strain LBA4404 and transformed into Arabidopsis WT or mutant plants by the floral-dip method(Clough & Bent, 1998). Approximately 30 T1 transgenic plants for each transgene were screened on MS medium supplemented with the appropriate antibiotics, and phenotypic analyses were performed in T2 or later generations.