Dual-luciferase assay
Protoplasts were isolated and the dual-luciferase assay was performed as our previously described paper (Han et al., 2017). Transformed protoplasts were incubated at room temperature for 20-22 h, and luciferase activities were measured using the dual-luciferase assay system (Dual-Luciferase Reporter Assay, Promega, United States) according to the manufacturer’s instructions. Firefly luciferase activity was normalized to Renilla luciferase activity. Three biological replicates were performed for all experiments.