Fig. S2 Electrophoretic mobility shift assay (EMSA) showing
binding of COL3 to the COL13 promoter in vitro.
Fig. S3 COP1 can interact with COL3, but not COL13. a COL3-CFP
and COP1-YFP co-localize to the nucleus in protoplasts in both light and
dark conditions. b FRET between CFP-COL3 and YFP-COP1 analyzed by
acceptor bleaching in the nucleus. The top panels in b show a
representative pre-bleach nucleus coexpressing YFP-COP1 and CFP-COL3
excited with either a 514- or a 405-nm laser in light and dark,
resulting in emission from YFP (yellow) or CFP (blue), respectively. The
bottom panels in b show the same nucleus after bleaching following
excitation with a 514- or a 405-nm laser. The relative intensities of
both YFP and CFP were measured once before and twice after bleaching, as
indicated in c and d. e COL13-CFP and COP1-YFP co-localize to the
nucleus in protoplasts in light and dark. f FRET between CFP-COL13 and
YFP-COP1 analyzed by acceptor bleaching in the nucleus. The top panels
in f show a representative pre-bleach nucleus co-expressing YFP-COP1 and
CFP-COL13 excited with either a 514- or a 405-nm laser in light and
dark, resulting in emission from YFP (yellow) or CFP (blue),
respectively. The bottom panels in f show the same nucleus after
bleaching following excitation with a 514- or a 405-nm laser. The
relative intensities of both YFP and CFP were measured once before and
twice after bleaching, as indicated in g and h.