Dual-luciferase assay
Protoplasts were isolated and the dual-luciferase assay was performed as
our previously described paper (Han et
al., 2017). Transformed protoplasts were incubated at room temperature
for 20-22 h, and luciferase activities were measured using the
dual-luciferase assay system (Dual-Luciferase Reporter Assay, Promega,
United States) according to the manufacturer’s instructions. Firefly
luciferase activity was normalized to Renilla luciferase activity. Three
biological replicates were performed for all experiments.