COL13 RNA accumulates to high levels in hypocotyls
By searching the gene expression information in The Arabidopsis
Information Resource (TAIR) database
(Klepikova, Kasianov, Gerasimov,
Logacheva, & Penin, 2016), we found that COL13(AT2G47890 )
highly expressed in hypocotyl. Quantitative PCR
(qPCR)
analysis confirmed that COL13 were expressed in most plant
organs, with a higher expression in hypocotyl and stem (Fig.1a). To
determine the spatial patterns of COL13 expression in more
detail, transgenic lines expressing GUS driven by the 2812 bpCOL13 promoter fragment were generated. As shown in Fig.1b, GUS
expression was predominantly active in hypocotyl.