Yeast assays
The yeast two-hybrid and three-hybrid assay were performed using a Clontech kit (PT3024-1 (PR973283)). For yeast two-hybrid assays, the bait vectors (pGBKT7 plus candidate genes) and prey vectors (pGAGT7 plus candidate genes) were transformed to Gold and Y187 yeast strain, respectively, then pick each one colony of them to do the mating, and spray the mating solution on SD/-Trp/-Leu/X-α-Gal/AbA (DDO/X/A) agar plates, the positive results were confirmed by growing them on SD/-Ade/-His/-Trp/-Leu/X-α-Gal/AbA (QDO/X/A) agar plates. For yeast three-hybrid assay, COP1-pGADT7 and COL3-COL13-pBbidge constructs were co-transformed into Gold yeast strain. Pick each colony and grow them in SD/-Leu/-Met/-Trp and SD/-Leu/-Trp solution, respectively. Normalized Miller Units were calculated as a ratio of α-galactosidase activity in yeast. For all yeast assays, we used empty vector as controls.