Hypocotyl Experiments
The light-response assays were performed as previously with some changes
(Datta et al., 2006). Plates were 4°C
treated for 72 hours and then moved to white light for 10 h to induce
uniform germination. After that, the plates were transferred to
different light conditions (Dark, white, red and blue light) and
incubated at 22℃ for 3-6 d for hypocotyl measurement. Red and blue
lights were generated by light emission diodes at 670 and 470 nm,
respectively (model E-30LED; Percival Scientific). The light intense is
around 100 umol/m2·s ppfd. The hypocotyl lengths of seedlings were
measured/counted using ImageJ software.