COL13 RNA accumulates at high levels in hypocotyls
By searching the gene expression information in the Arabidopsis
Information Resource (TAIR) database (Klepikova, Kasianov, Gerasimov,
Logacheva, & Penin, 2016), we found that COL13(AT2G47890 )
is highly expressed in the hypocotyl. Quantitative PCR
(qPCR)
analysis confirmed that COL13 was expressed in most plant organs,
with higher expression in the hypocotyl and stem (Fig. 1a). To determine
the spatial patterns of COL13 expression in more detail,
transgenic lines expressing GUS driven by the 2812 bp COL13promoter fragment were generated. As shown in Fig. 1b, GUS expression
was predominantly in the hypocotyl.