Fig. S2 Electrophoretic mobility shift assay (EMSA) showing binding of COL3 to the COL13 promoter in vitro.
Fig.S3 Hypocotyl lengths of the indicated genotypes were measured at the 5th day. Error bars indicate SD (n >15). Asterisks indicate that hypocotyl lengths in 35S:COL-HA and 35S:COL13-GFP are significantly different with Col-0 under red light (P < 0.05).
Fig. S4 COP1 can interact with COL3, but not COL13. a COL3-CFP and COP1-YFP co-localized to the nucleus in protoplasts under both light and dark conditions. b FRET between CFP-COL3 and YFP-COP1 analyzed by acceptor bleaching in the nucleus. The top panels in b show a representative pre-bleach nucleus co-expressing YFP-COP1 and CFP-COL3 excited with either a 514 or a 405 nm laser in light and dark, resulting in emission from YFP (yellow) or CFP (blue), respectively. The bottom panels in b show the same nucleus after bleaching following excitation with a 514 or a 405 nm laser. The relative intensities of both YFP and CFP were measured once before and twice after bleaching, as indicated in c and d. e COL13-CFP and COP1-YFP co-localized to the nucleus in protoplasts in light and dark. f FRET between CFP-COL13 and YFP-COP1 analyzed by acceptor bleaching in the nucleus. The top panels in f show a representative pre-bleach nucleus co-expressing YFP-COP1 and CFP-COL13 excited with either a 514 or a 405 nm laser in light and dark, resulting in emission from YFP (yellow) or CFP (blue), respectively. The bottom panels in f show the same nucleus after bleaching following excitation with a 514- or a 405-nm laser. The relative intensities of both YFP and CFP were measured once before and twice after bleaching, as indicated in g and h.