Plant transformation
Constructs in binary vectors were introduced into theAgrobacterium tumefaciens strain LBA4404 and transformed intoArabidopsis wild type (WT) or mutant plants by the floral-dip
method (Clough & Bent, 1998). Approximately 30 T1 transgenic plants for
each transgene were screened on MS medium supplemented with the
appropriate antibiotics, and phenotypic analyses were performed on T2 or
later generations.