Fig. S2 Electrophoretic mobility shift assay (EMSA) showing
binding of COL3 to the COL13 promoter in vitro.
Fig.S3 Hypocotyl lengths of the indicated genotypes were
measured at the 5th day. Error bars indicate SD
(n >15). Asterisks indicate that hypocotyl lengths in
35S:COL-HA and 35S:COL13-GFP are significantly different with Col-0
under red light (P < 0.05).
Fig. S4 COP1 can interact with COL3, but not COL13. a COL3-CFP
and COP1-YFP co-localized to the nucleus in protoplasts under both light
and dark conditions. b FRET between CFP-COL3 and YFP-COP1 analyzed by
acceptor bleaching in the nucleus. The top panels in b show a
representative pre-bleach nucleus
co-expressing
YFP-COP1 and CFP-COL3 excited with either a 514 or a 405 nm laser in
light and dark, resulting in emission from YFP (yellow) or CFP (blue),
respectively. The bottom panels in b show the same nucleus after
bleaching following excitation with a 514 or a 405 nm laser. The
relative intensities of both YFP and CFP were measured once before and
twice after bleaching, as indicated in c and d. e COL13-CFP and COP1-YFP
co-localized to the nucleus in protoplasts in light and dark. f FRET
between CFP-COL13 and YFP-COP1 analyzed by acceptor bleaching in the
nucleus. The top panels in f show a representative pre-bleach nucleus
co-expressing YFP-COP1 and CFP-COL13 excited with either a 514 or a 405
nm laser in light and dark, resulting in emission from YFP (yellow) or
CFP (blue), respectively. The bottom panels in f show the same nucleus
after bleaching following excitation with a 514- or a 405-nm laser. The
relative intensities of both YFP and CFP were measured once before and
twice after bleaching, as indicated in g and h.