FIGURE LEGENDS
Figure 1. Neighbour-joining phylogram showing the placement of Nakazawaea atacamensis sp. nov sp. nov. within the genus . The tree was built based on the sequences of the D1/D2 domains of the LSU rRNA gene. The alignment was performed with MUSCLE. Pachysolen tannophilus was used as an outgroup. The distance metric is the number of substitutions. Bar, Substitutions per site.
Figure 2. Differential interference contrast micrograph of budding cells of Nakazawaea atacamensis sp. nov. Micrograph of budding cells grown in YPD broth after 3 days at 25°C. Bars: 5 μm. This image was obtained using differential interference contrast (DIC) microscopy.
Figure 3. Maximum likelihood phylogenetic tree using whole-genome sequences. A concatenated alignment of 2,422 single-copy orthologs was used to construct the ML tree. P. tannophilus was used as an outgroup. Branch lengths denote amino acid substitutions per site.
Figure 4. N. atacamensis genome analyses. A. KEGG distribution based on gene prediction using KofamKOALA. B. KEGG enrichment analysis of the carbohydrate metabolism pathways. In both figures the x-axis depicts the number of genes on each pathway identified in the N. atacamensis genome.
Figure 5. Fermentation phenotypes in N. atacamensis . A . Growth rate under glucose, fructose and sucrose 2% w/v, and glucose 20% w/vas carbon sources. B . Growth rates under medium supplemented with glucose 2% w/v and ethanol at 6 and 8% v/v. Fermentation results in Synthetic Wine Must (SWM) with a YAN concentration of 300 mg/mL. C . CO2 loss, D . Sugar consumption, E. Ethanol production and F . Glycerol production. EC1118 (grey dots) and N. atacamensis(orange dots).