FIGURE LEGENDS
Figure 1.
Neighbour-joining phylogram showing the placement of Nakazawaea
atacamensis sp. nov sp. nov. within the genus . The tree was built
based on the sequences of the D1/D2 domains of the LSU rRNA gene. The
alignment was performed with MUSCLE. Pachysolen tannophilus was
used as an outgroup. The distance metric is the number of substitutions.
Bar, Substitutions per site.
Figure 2. Differential interference contrast micrograph of
budding cells of Nakazawaea atacamensis sp. nov. Micrograph of
budding cells grown in YPD broth after 3 days at 25°C. Bars: 5
μm. This image was obtained using differential interference contrast
(DIC) microscopy.
Figure 3. Maximum likelihood phylogenetic tree using
whole-genome sequences. A concatenated alignment of 2,422 single-copy
orthologs was used to construct the ML tree. P. tannophilus was
used as an outgroup. Branch lengths denote amino acid substitutions per
site.
Figure 4. N. atacamensis genome analyses. A. KEGG
distribution based on gene prediction using KofamKOALA. B. KEGG
enrichment analysis of the carbohydrate metabolism pathways. In both
figures the x-axis depicts the number of genes on each pathway
identified in the N. atacamensis genome.
Figure 5. Fermentation phenotypes in N.
atacamensis . A . Growth rate under glucose, fructose
and sucrose 2% w/v, and glucose 20% w/vas carbon sources. B .
Growth rates under medium supplemented with glucose 2% w/v and ethanol
at 6 and 8% v/v. Fermentation results in Synthetic Wine Must (SWM) with
a YAN concentration of 300 mg/mL. C . CO2 loss, D .
Sugar consumption, E. Ethanol production and F .
Glycerol production. EC1118 (grey dots) and N. atacamensis(orange dots).