Supporting Information
Figure S1. Average protein similarity of transporters in different plant clades.
Figure S2. Average protein similarity in different clades of gene families in different plant clades.
Figure S3. Phylogenetic tree of SPX-MFSs and PHT1s from algae to angiosperms
Figure S4. Conserved domains in SPX-MFSs.
Figure S5. Conserved domains in PHT1s.
Figure S6. Total root length of three barley genotypes in response to Al stress.
Figure S7. Metabolite profiles and metabolic pathways in root tip of barley XZ29 and XZ9 under Al treatment.
Figure S8. Correlation analysis of primary metabolites.
Figure S9. The effect of P addition on primary metabolites in barley root tips treated with 72 h of Al treatment.
Figure S10. Fluorescence imaging of Al distribution in root cross-sections of XZ29 and XZ9.
Figure S11. Secondary ion mass spectrometry (SIMS) of Al distribution in root elongation zone of XZ29 and XZ9. Al distribution in root elongation zone of XZ29 (a-d) and XZ9 (e-h) after 2, 6, 24, and 72 h of 5 μM Al treatments.
Table S1. Primers for PCR and qRT-PCR.
Table S2. List of gene families related with plant Al tolerance.
Table S3. Protein similarity of Al tolerance-associated genes/ gene families in 41 species of land plants and algae with E value < 10-5.
Table S4. Gene number of Al tolerance-associated genes/ gene families in 41 species of land plants and algae with E value < 10-5.
Table S5. Detection of the 1-kb insertion in HvAACT1upstream region in 110 Tibetan wild barley accessions and a barley cultivar Dayton.
Table S6. The changes of primary metabolites in response to 6 to 72 h of Al treatments.
Table S7. The changes of primary metabolites in response to 2 to 24 h of P addition after 72 h Al treatment.
Table S8. Gene expression of Pi transporter genes in XZ-29 and XZ-9 after 0.5, 2, 6, 12 and 24 h of 5 μM Al treatments.