Statistical analysis
All data were expressed as mean ± standard deviation. The intra-assay
results were compared using t-tests. *P <0.05 was
considered to be statistically significant difference. Five medium
samples were taken for each experiment, and each experiment was repeated
at least two times, using blood samples from different individuals. Data
analysis was performed using Graph Prism 6.0 software (RRID:SCR_002798,
La Jolla, CA, USA).