EXPERIMENTAL APPROACH
In this study, we use freshly extracted human leukocytes to study
effects of a recombinant human intestinal alkaline phosphatase (recIAP)
on secretion of TNF-ɑ and IL-6 by the leukocytes in absence of LPS.
Physiological substrates of alkaline phosphatase and their
dephosphorylated products at neutral pH including ATP, ADP, AMP and
adenosine were employed to investigate their effects on secretion of
TNF-ɑ by the leukocyte.