bIAP, as well as recIAP, inhibit the secretion of TNF - α and
IL-6
To study the effect of extracted
bovine intestinal alkaline phosphatase (bIAP) on leukocyte secretion of
TNF-α and IL-6, bIAP was subjected to the same cell culture models as
recIAP. The results showed that bIAP (5U ml-1)
significantly inhibit human leukocytic secretion of TNF-α and IL-6 in
the presence of LPS (0.5ng ml-1) (Figure 4 black bar).
bIAP also exhibited a significant inhibitory effect on TNF-α and IL-6 in
the absence of LPS (Figure 4 white bar). The inhibitory effect of bIAP
and recIAP on TNF-α and IL-6 secretion which has been demonstrated in
our experiment reflects the in vivo function of IAP expressed on
the surface of intestinal mucosa to act in an anti-inflammatory capacity
against fecal endotoxin content released by E. coli (Poelstra,
Bakker, Klok , Hardonk, Meijer, 1997; Kaliannan, 2013; Narisawa, 2003;
Peters, 2016a; Lukas,2013).