Detection of intestinal alkaline phosphatase activity
Alkaline phosphatase activity was measured using a phenyl disodium
phosphate colorimetry kit (TE0007, Beijing Regen Biotechnology Co.,
Ltd.). Phenyl disodium phosphate is hydrolyzed by alkaline phosphatase
under alkaline conditions to form free phenol and phosphoric acid. Under
basic conditions, phenol is combined with amino antipyrine and oxidized
to form red quinone structures with different shades. The absorbance was
measured at 510 nm and the alkaline phosphatase activity level was
calculated by colorimetric analysis. An active unit (U
L-1) is defined as 100 milliliters of sample at 37°C
for 15 minutes of incubation with benzene disodium phosphate in order to
produce 1 milligram of phenol.