Figure 6: Vit D3 rescued CD4+ T cells inhibited PD1+CD4+ T cells
in the absence of ST2 after RV infection of lung cells
(a) Experimental design. WT B6 n=4. ST2-/- n=2. IL33-/- n=4. (b)
Analysis of CD4+ lymphocytes from wild-type and ST2-/- mice, infected
with RV1b and afterwards treated with 1a,25 DihodroxyvitaminD3 (=VitD3)
and/or OVA in vitro. p=0,029. p=0,009. p=0,026. Two-tailed Student’s T
test. p= 0,00028. p=0,022. p=0,028. Two-tailed Student’s T test.
(c) PD1+CD4+ cells gated on CD4+lymphocytes, from wild-type (n=4) and
ST2-/- (n=2) mice, infected with RV1b and afterwards treated with 1a,25
DihodroxyvitaminD3 (=VitD3) and/or OVA in vitro. p=0,009. p=0,048.
p=0,045. Two-tailed Student’s T test.