The lysogenization of the non-O157 Escherichia coli strains by stx-converting bacteriophage phi24B is associated with the O antigen loss and reduced fitness

Golomidova A.K.^a, Efimov A.D.^a, Kulikov E.E.^a,b, Kuznetsov A.S.^a,c , Letarov A.V.^a,c*

^aWinogradsky Institute of Microbiology RC Biotechnology RAS, Moscow, Russia

^bPhystech School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Moscow, Russia

^cFaculty of Biology, Lomonosov Moscow State University, Moscow, Russia

^*Prospekt 60-letiya Oktyabrya 7 bld. 2., 117312, Moscow, Russia. letarov@gmail.com

The lysogenization of the non-O157 Escherichia coli strains by stx-converting bacteriophage phi24B is associated with the O antigen loss and reduced fitness

Acquisition of new prophages that are able to increase the bacterial fitness by lysogenic conversion is believed to be important strategy of bacterial adaptation to changing environment. However, in contrast to the factors determining the range of bacteriophage lytic activity, little is known about the factors that define the lysogenization host range. Bacteriophage phi24B is the paradigmal model of stx-converting phages, encoding the toxins of the Shiga-toxigenic E. coli(STEC). This virus has been shown to lysogenize the wide range ofE. coli strains that is much broader than the range of the strains supporting its lytic growth. Therefore, phages produced by the STEC population colonizing the small intestine are potentially able to lysogenize symbiotic E. coli in the hindgut, and these secondary lysogens may contribute to the overall patient toxic load and to lead to the emergence of new pathogenic STEC strains. We demonstrate, however, that O antigen effectively limit the lysogenization of the wild E. coli strains by phi24B phage. The lysogens are formed from the spontaneous rough mutants and therefore have increased sensitivity to other bacteriophages and to the bactericidal activity of the serum if compared to their respective parental strains.

Keywords: stx-converting bacteriophages; shiga-toxigenic E. coli ; LPS; O antigen; bacteriophage adsorption; temperate bacteriophages