Seed length and Spikelet imaging
Seed length was measured using a Leica MZFLIII fluorescence
stereomicroscope. About 12 seeds were measured for each plot, and the
mean value was recorded. Spikelet images for each line were recorded
using Leica MZFLIII stereomicroscope as well.
Height dynamics Average plant height in each plot was measured
from 53 DAS up to 77 DAS (eight times). Height (H) was measured at three
different locations along the plot, from the ground up to maximal plant
height (which could not be absolutely defined as the panicle tips). As
panicles emerged (and during grain filling) the fraction of the plot
which exhibited lodging was scored (F), as well as the height of the
lodged fraction (LH). p Plant heights were averaged as follows:
Avg plot plant height = (Erect fraction * H) + (Lodged fraction * LH)
Maximal plot height (MPH) was the maximal height the plot had reached,
regardless of the lodging fractions.