Seed and Spikelet phenotyping
AGW of the brown segregated lines 53-1-B and 53-3-B was significantly higher as compared to their white counterparts (Table 7). The commercial cultivar exhibited midrange values of AGW. Similar pattern was obtained for SL, where this time all three brown segregated lines exhibited significantly higher averaged values as compared to their white counterparts (Table 7).
Coloration of the Spikelet lemma was also documented (Fig. 3). The white commercial cultivar lemma were gray-purplish and highly transparent. 44A-163-B exhibited a dark-purple coloration and was transparent as well. 44B-163-W exhibited bright pink lemma with whitish-gray outer borders and veins. The brown seeded line of 53-1 exhibited much less pink coloration (mainly at the outer borders) compared to their white counterparts, which had bright pink lemma with white gray outer borders and veins. The second brown line of 53-2 exhibited purple coloration at the outer border of the lemma as compared to their white counterparts which were pink - not purple. The third brown line of 53-3 also exhibited purple coloration at the outer borders of the lemma as compared to the white counterparts, which had whitish-gray lemma (similar to the white cultivar in terms of coloration).