Supplementary material
Figure S1. Scatterplots between per-sample richness of taxonomic units from all metabarcoding treatments.
Figure S2. Venn diagrams of the diatom genera relations between pipelines (ESVs, 97% OTUs, 95% OTUs).
Figure S3 . Scatterplots between per-sample richness of taxonomic units from all metabarcoding treatments and microscopy.
Figure S4 . Scatterplots between relative abundance of genera from metabarcoding treatments and microscopy. Relative abundance is calculated as based on the number of sequences and valves of the matching genera between metabarcoding and microscopy data sets, respectively.
Figure S5 . Most important community assembly variables as selected by Random Forest analyses. Graphs a-d) represent analyses results for 11 corresponding samples from microscopy and HTS 0.5 data sets. Graphs from e) to h) represent 14 corresponding samples from microscopy and HTS 10 data sets.
Table S1. Sediment samples used in this study, and their measured environmental parameters.
Table S2. Primer used in this study. PCR primers = adapter+tag+pad+link+primer.
Table S3. ESV/OTU (and genus level) occurrence tables per samples for sequence data, and species occurrence table for microscopy data.
Table S4. Marginal tests for all data sets. Included variables are the ones selected as most important by Random Forest analyses.