Author contributions
EN and VB designed the study, performed all statistical analyses, and
drafted the manuscript. Adult frogs were captured and morphologically
sexed, and DNA samples were taken from them by VV, BÜ, and VB. Eggs were
collected by VB, BÜ, NU, VV and EN. Froglets in the laboratory were
raised by VB, ZM, NU, EN and VV. Dissection and phenotypic sexing was
performed by NU and VV, and histological samples were prepared and
analysed by KKL. DNA extractions were performed by EN. Putatively
sex-linked sequences were identified by DLJ, out of which loci were
chosen by EN and VB for further analyses based on genome data provided
by DLJ. PCR primers were designed, PCR conditions were optimized and
PCR-based sexing was performed by EN, while HRM primers were designed
and HRM conditions were optimized by OIH and ZG, and HRM-based sexing
was performed by ZG. Genotype analyses were performed by EN, and
geoinformatics analyses were performed by NU. All authors proofread the
manuscript, EN, VB, DLJ and BÜ revised the first draft, and all authors
gave final approval for publication.