Locus Accession number Primer Primer sequence µl in mix a T (°C) PCR ID X/Y (bp) Y (bp)
Rds1 MT358850- MT358851 Rds1-F F: GACAGGATAGATATGTAAATAGTAGC 1.3 65-63 TD sex
Rds1-R R: GATACCCTGGCCTGAATTTCC 0.1 PCR 207 97
Rds1-Y-R R: GGCCTGGTTAGTTGGTATGTA 2.5 1
Rds2 MT358852- MT358853 Rds2-F F: CGACCCCCAGGTTAAGAATCA 1.3 sex
Rds2-R R: CCGGTGCATGAGTCTATCCC 0.6 70 PCR 507 341
Rds2-Y-R R: AGCGGGCAGCACTAACTTGT 0.7 2
Rds3 b MT358846- MT358849 Rds3-F F: TGGTTGTAACATGACAAAATGTGGA 0.2 70-65 TD sex
Rds3-Y-F F: CAAGGCACTGTACCTGGTT 2 PCR 218 166
Rds3-R R: GTCCATGTCAATGGATGCTGC 1.5 1
Rds3-HRM-F F: AAAGTTCTAGGGGTATGAATACTTTTT 1 62 sex 99 -
Rds3-HRM-R R: GGGACCCCAGAAGTAGAGTATTG 1 HRM