Na: number of individuals; Ho: observed
heterozygosity; He: expected heterozygosity under
Hardy–Weinberg equilibrium; TA°C: annealing temperature.
p-values for the Hardy–Weinberg Equilibrium test, significance
threshold adjusted using sequential Bonferroni correction: *p ≤ 0.05,
***p ≤ 0.001, ns = not significant .
Conclusion
Illumina MiSeq sequencing technology enables us to develop 30 pairs of
microsatellites primers based on P. erinaceus populations.
Seventeen among them were polymorphic in fifteen (15) West African
locations and they provide the first set of microsatellite markers
dedicated for P. erinaceus. These microsatellite markers will be
useful for the characterization of genetic diversity and analysis of
genetic structure for P. erinaceus populations.
Data Accessibility
The data have been deposited with links to BioProject accession number
PRJNA604893 in the NCBI BioProject database
(https://www.ncbi.nlm.nih.gov/bioproject/)
17 Primers designed and evaluated: end of text (Table 2).
ORCID
Benziwa Nathalie Johnson ID:https://orcid.org/0000-0003-0018-8085
Conflicts of Interest
The authors have no conflicts of interest to declare.
Authors contributions
All authors contribute significantly to the present study and to the
revision of the manuscript. Manuscript writing: B.N.J., with support
from M.L.A.Q., G.C., L.C-K., H.V., A.D.K and K.K. DNA extractions, PCR
running, DNA sequencing, microsatellites primers identification and
selection: B.N.J. and H.V.
Genetic analyses and statistical treatments: B.N.J., G.C., L.C-K.
Coordination of data sampling: H.V. M.L.A.Q., K.A., A.D.K. and K.K.
Trees sampling in the field: B.N.J. and K.N.S.
Leading research project: M.L.A.Q.
Acknowledgements
This research was financially supported by: Sud Expert Plantes
Développement Durable (SEP2D) program [AAP1-45], Agence
Universitaire de la Francophonie (AUF) [DRAO-1448-2019-02],
Programme d’Appui à la Lutte contre le Changement Climatique : PALCC
–AMCC+ [DCI-ENV/2016/39289 Devis programme multi annuel
n°DCI-ENV-2017/387-416], Service de Coopération et d’Action Culturelle
(SCAC) and Campus France, CIRAD for the “Actions Incitatives-Soutien
aux doctorants” Program, and genotyping platform of Agap unit managed
by Pierre Mournet.
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