FIGURES CAPTIONS
Fig. 1. Experimental sampling design.
Fig. 2. MOTUs richness across the different treatments (control: immediate extraction; T2: extraction after 6h at room temperature; T3: extraction after three days at 4°C; T4: immediate preservation in tubes with silica gel, extraction after 21 days; T5: preservation in tubes with silica gel after 6h at room temperature, extraction after 21 days) before (left) and after (right) removing MOTUs with frequency < 1% in one sample.
Fig. 3. Plots of non-metric dimensional scaling showing dissimilarities of communities among the three habitats: broadleaved forest (black); grassland (green); vegetated riverbank (blue). Each plot shows the results of metabarcoding analysis based on soil samples subjected to five different treatments.
Fig. 4. Procrustes correlation between communities obtained from metabarcoding analyses based on soil samples across environmental conditions subjected to five sample treatments (control: immediate extraction; RT+6h: extraction after 6h at room temperature; 4°C: extraction after three days at 4°C; silicagel: immediate preservation in tubes with silica gel, extraction after 21 days; silicagel+6h: preservation in tubes with silica gel after 6h at room temperature, extraction after 21 days. All correlation coefficients are highly significant (all P < 0.0001).
Fig. 5. Guidelines for improving monitoring strategies with eDNA from soil.