Bacteriological examination and identification
Phenotypically, all Brucella isolates (n=41) proved to beB. melitensis bv3 by fitting the identification scheme of Alton et al. (1988) and OIE, (2018). AMOS-PCR showed B. melitensis  specific band of 731 bp. Thirty-one isolates were obtained from 28 sheep and 3 goats (the preferential host), and ten isolates were obtained from nine cattle and one buffalo (non-preferential  hosts) as, shown in Table 2.