Figure 2: Supergene-mediated differential gene expression. (A)
Volcano plot of gene expression differences between SB/SB andSB/Sb gynes, independent of organ and social-environment effects
(multifactorial-model). Aqua dots represent statistically significant
differences (FDR < 0.01). (B) Volcano plot of gene expression
differences between SB/SB and Sb/Sb gynes. (C) UpSet plot
showing organ-specific patterns of differential gene expression
associated with differences in social chromosome genotype in
polygyne-reared gynes. The pattern of genes common to different
comparisons is indicated by the dots below the vertical bar plot.
Numbers in parentheses indicate the genes remaining in a category after
elimination of cusp cases (genes with FDR = 0.01-0.1 in other contexts,
described in Methods). (D) Heatmap of the distribution of differentially
expressed genes across the linkage-mapped genome in polygyne-reared
gynes. The region spanned by the lg16 Sb supergene is marked in
green. Labels to the right of the heatmap refer to candidate genes in
each of the categories described in panel C.