Supplementary Figure S5: Spatial patterns of differentially expressed genes (DEGs) within the supergene in the Yan et al. 2020 SBassembly. The social chromosome (chr16) physical map is shown at the bottom, with the supergene location marked in green, blue, and red above it. Each color represents a region generated by a particular inversion. Red indicates the first inversion, blue the second, and green the third. Additionally, there is a small purple region on the right end of the supergene that was present in both the first and second inversion. Each track represents a differential expression analysis with GLM comparisons describing the comparisons using a multifactorial model. Dots indicate significant DEGs (FDR < 0.01) with genes upregulated in the presence of Sb marked by aqua dots and genes upregulated inSB/SB marked by yellow dots. The Y-axes indicate log₂ fold-difference in expression and the size of the dots indicates FDR-corrected P-value. Region 1, demarcated by the yellow line at the top of the plot, delineates a hotspot of SB- linked expression; Region 2, demarcated by a blue line, delineates a hotspot ofSb -linked expression.