CD183+CCR7+CD45RA- CD8+Treg inhibit B cell proliferation
Purified B cells were first labeled with CFSE and then co-cultured
without or with sorted CD183+CCR7+CD45RA- CD8+Treg in B: CD8Treg ratio
of 1:1, 1:1/2 and 1:1/4 and activated with anti-CD40 and CpG for 5 days
and proliferation was assayed for CFSE dilution. A representative flow
cytometry histogram is shown in Figure 1A and cumulative data from 6
different individuals are shown in Figure 1B. Data are expressed as mean
±sem. A significant inhibitory effect was observed only at B: CD8Treg
ratio of 1:1.