3.5.1 Vesivirus
Feline calicivirus, genus Vesivirus, familyCaliciviridae, is a highly contagious pathogen of domestic cats and other Felidae (Gaskell et al., 2004). It typically causes self-limiting upper respiratory tract disease (URTD) (Gaskell et al., 2004). Recently enteric-adapted Feline calicivirus strains have been isolated from cats with enteritis in single-agent infections or together with other enteric viruses (Di Martino et al., 2020). Here,Feline calicivirus contigs were significantly more frequent in FPV-cases (47.8%, 11/23) compared to healthy controls (13.9%, 5/36) (p= 0.0067, Figure 2). With the exception of 2,889 and 6 reads from the FPV-case and healthy control libraries, respectively, no Feline calicivirus contigs were identified as Feline calicivirusvaccine strain F9. In contrast, 409,597 reads (320 RPM) mapped to theFeline calicivirus contigs identified in the FPV-case libraries (Table 1). Feline calicivirus contigs identified in the other nine FPV-case libraries ranged from 203 to 7864bp. In the control libraries the longest Feline calicivirus contig was 815bp. The phylogenetic analysis of the full genome and capsid protein shows the enteric sequences in this study do not group with the other identified enteric sequences in the phylogeny, instead forming a clade with other sequences from Australia (Figure 5).