2.6 Vaccine PCR and sequencing
The non-structural (NS) and VP2 genes from the FPV Feligen RCP modified live vaccine (Virbac, France) were amplified and sequenced as previously described (Pérez et al., 2014; Van Brussel et al., 2019). Amplicons were sequenced at the AGRF (Melbourne, Australia) and Geneious version 2020.2.5 was used to determine the consensus contig of the NS and VP2 based on the forward and reverse sequence.