Offspring fat analysis
We dried pupae in an incubator at 70°C for 48 hours, and then recorded
their dry weight before placing them in ether inside a metal tray. We
changed the ether each day for three days. On the fourth day, samples
were again dried for 48 hours in an incubator at 70°C and then
re-weighed to estimate the residual dry weight. We calculated the
difference between the dry weight and residual dry weight to estimate
the fat previously described (Buxton & Lewis 1934; Phelps 1973).