Figure 3: Side by side characterization of baculoviral reagents derived from HR or Tn7 systems. a) Characterization of BEV clones where genome stability is monitored by qPCR quantification using specific amplicons along serial passages of amplification. BEVs are derived from the HR- or Tn7-based system with a total of 5 clones per system. The ratio bac/amplicon is calculated and plotted to compare clones (n = 1 or 2). Mean ± SD. b) Western blot analysis of “Cap” proteins (VP1, VP2 and VP3) after serial steps of BEV rep2/cap8 clonal amplification derived from HR and Tn7 systems.