Figure 4: Recombinant AAV production using both HR and Tn7
derived baculoviral reagents.
a) Total vector genomes (vg) quantification by qPCR at harvest
time or after purification for AAV2/8-GFP (n = 3) or AAV2/anc80-GFP (n =
2) preparations. Titers were normalized for a standard production volume
equal to 125ml. b) Quantification of total AAV2/8-GFP viral
particles (vp) by ELISA assay or total viral genomes (vg) by qPCR in
clarified crude bulk (n = 3). c) Left: quantification of
infectious units (iu) of AAV particles by ICA assay in purified AAV8-GFP
(n = 3) and AAVanc80-GFP (n = 2) batches; Right: representation of
infectivity expressed as a ratio “vg/IU” for the indicated constructs.
Mean ± SD.