Figure 2: Generation of baculoviral reagents with HR system.
Characterization of five BEV rep2cap8 clones (P1) generated with HR
system and derived from circular or digested (= linearized) “pBac”
donor plasmid. a) Infectious titers for each clone using the
cell size assay method (n = 1). b) Viral genome qPCR
quantification using specific “bac” and “rep” amplicons. A ratio
(bac/rep) is calculated and plotted to compare clones altogether (n =
2). Mean ± SD. c) Western blot analysis of “Rep” proteins
(Rep78 and Rep52) and “Cap” proteins (VP1, VP2 and VP3) after serial
steps of clone amplification (P1, P5 and P10).