Figure 3: Side by side characterization of baculoviral reagents
derived from HR or Tn7 systems. a) Characterization of BEV
clones where genome stability is monitored by qPCR quantification using
specific amplicons along serial passages of amplification. BEVs are
derived from the HR- or Tn7-based system with a total of 5 clones per
system. The ratio bac/amplicon is calculated and plotted to compare
clones (n = 1 or 2). Mean ± SD. b) Western blot analysis of
“Cap” proteins (VP1, VP2 and VP3) after serial steps of BEV rep2/cap8
clonal amplification derived from HR and Tn7 systems.