Figure 5: rAAV characterization derived from HR and Tn7 reagents.
a) Total gentamycin copies quantified by qPCR in purified AAV8-GFP (n = 3) or AAVanc80-GFP (n = 2) batches and normalized to 1012 total viral genome (vg). Mean ± SD. b)Top: western blot analysis of “Cap” proteins (VP1, VP2 and VP3) for different replicates of purified AAV preparations carrying indicated constructs; Bottom: silver staining analysis of total proteins and purity for different replicates of purified AAV preparations carrying indicated constructs.