Genotyping procedures
For both species, a panel of 9-10 neutral microsatellite markers were
amplified in the selected samples (Table S1) using a multiplex primary
PCR followed by individual nested PCRs as previously described (Anderson
et al., 2000; Jennison et al., 2015; Koepfli et al., 2013; Schultz et
al., 2010). For P. falciparum , samples were genotyped at nine
previously validated and commonly used, putatively neutral,
microsatellite loci including TA1, TAA60, Polya, ARA2, Pfg377,
TAA87, PfPK2, TAA81 and 2490 (Anderson et al., 2000; Schultz et
al., 2010). For P. vivax , 10 putatively neutral microsatellites
were genotyped as previously described: MS1, MS2, MS5, MS6, MS7,
MS9, MS10, MS12, MS15 , and MS20 (Jennison et al., 2015; Koepfli
et al., 2013). All PCR products were sent to a commercial facility for
fragment analysis on an ABI3730xl platform (Applied Biosystems) using
the size standard LIZ500. Primers used were the same for all datasets
(Jennison et al., 2015; Schultz et al., 2010).