Figure 1. Biocatalytic cascade for the synthesis of
polycaprolactone monomers. The cascade is composed of a Cytochrome P450
monooxygenase (Cyp), a cyclohexanol dehydrogenase (CDH), and a
cyclohexanone monooxygenase (CHMO) for the production of ε-caprolactone
(ε-CL) [16]. Optionally, a lactonase (Lact)
catalyzes the ring-opening reaction to yield 6-hydroxyhexanoic acid
(6HA).