4.3 Conclusion
In this study, we developed the strain P. taiwanensis VLB120 (pSEVA_6HA_2) that expresses 6 genes encoding 4 enzymes able to fully convert 5 mM cyclohexane to the PCL monomer 6HA. Accumulation of intermediates and byproducts was successfully prevented, and a high cascade activity was achieved. The constructed orthogonal pathway/ cascade also can serve as a template to be amended by additional enzymes to synthesize nylon monomers such as adipic acid and 6-aminohexanoic acid. This in combination with their solvent tolerance and versatility regarding reactor setups – including biofilm approaches[52] – qualify VLB120 strains harboring pSEVA_CL_2 or pSEVA_6HA_2 as promising platform organism.