3.3 Lamin A and trf2 co-immunoprecipitation analysis
To study the interaction between lamin A with trf2, immunoprecipitation
was performed. Briefly, protein lysate was prepared from human dermal
fibroblast (HDF) cells and immunoprecipitated with trf2 antibodies and
immunostained with lamin A antibodies. The data was shown in the (Figure
1D), which indicates that lamin A signals was clearly visualized in the
membrane. This indicates that lamin A has strong interaction with trf2.
Similarly, we performed co-immunoprecipitation with trf2 antibody and
stained with lamin A antibody and vice versa. Results for
co-immunoprecipitation was shown in the (Figure 1E), indicates the
presence of lamin A and trf2 signals in the membrane, clearly shows that
lamin A and trf2 have strong interaction among them. Further to validate
the data, we have performed immunofluorescence assay.