3.4 Immunofluorescence analysis
Further to study the interaction of lamin A and trf2 inside the fibroblast cells, we performed immunofluorescence assay with lamin A and trf2 specific antibodies. Briefly, fibroblast cells were fixed and stained with lamin A and trf2 antibodies. Both, lamin A and trf2 signals were neatly visualized under fluorescence microscope as red and green fluorescence, respectively (data not shown). Upon close observation, it was noted that trf2 signal was closely nearest to the lamin A signal. Hence, based on the immunofluorescence data, it is confirmed that lamin A has strong interaction with trf2.