3.4 Immunofluorescence analysis
Further to study the interaction of lamin A and trf2 inside the
fibroblast cells, we performed immunofluorescence assay with lamin A and
trf2 specific antibodies. Briefly, fibroblast cells were fixed and
stained with lamin A and trf2 antibodies. Both, lamin A and trf2 signals
were neatly visualized under fluorescence microscope as red and green
fluorescence, respectively (data not shown). Upon close observation, it
was noted that trf2 signal was closely nearest to the lamin A signal.
Hence, based on the immunofluorescence data, it is confirmed that lamin
A has strong interaction with trf2.