2.6 Immunofluorescence
Cultured HDF cells were fixed with ice-cold methanol followed by permeabilization with Triton X-100 and blocked with 1% BSA (Bovine Serum Albumin). After blocking, the cells were incubated with primary antibody (anti-lamin A and anti trf2) overnight, washed with 1X PBS and incubated with fluorophore conjugated secondary antibodies. Slides were mounted and observed under EVOS fluorescence microscope with DAPI as a counterstain.