2.9 Lamin A and trf2 interaction by Immunostaining
Wild and mutant transfected cells were grown on a coverslip in 6 well
plates. Cells were fixed with ice-cold methanol followed by
permeabilization with Triton X-100 and blocked with 1% BSA (Bovine
Serum Albumin). After blocking, cells were incubated with primary
antibodies (anti-lamin A and anti TRF2). Coverslip was washed with 1X
PBS and incubated with fluorophore conjugated secondary antibodies,
counterstained with DAPI. Coverslips were mounted and observed under
EVOS epi-fluorescence microscope.