2.6 Immunofluorescence
Cultured HDF cells were fixed with ice-cold methanol followed by
permeabilization with Triton X-100 and blocked with 1% BSA (Bovine
Serum Albumin). After blocking, the cells were incubated with primary
antibody (anti-lamin A and anti trf2) overnight, washed with 1X PBS and
incubated with fluorophore conjugated secondary antibodies. Slides were
mounted and observed under EVOS fluorescence microscope with DAPI as a
counterstain.