3.3 Lamin A and trf2 co-immunoprecipitation analysis
To study the interaction between lamin A with trf2, immunoprecipitation was performed. Briefly, protein lysate was prepared from human dermal fibroblast (HDF) cells and immunoprecipitated with trf2 antibodies and immunostained with lamin A antibodies. The data was shown in the (Figure 1D), which indicates that lamin A signals was clearly visualized in the membrane. This indicates that lamin A has strong interaction with trf2. Similarly, we performed co-immunoprecipitation with trf2 antibody and stained with lamin A antibody and vice versa. Results for co-immunoprecipitation was shown in the (Figure 1E), indicates the presence of lamin A and trf2 signals in the membrane, clearly shows that lamin A and trf2 have strong interaction among them. Further to validate the data, we have performed immunofluorescence assay.