3.2 Interaction of lamin A with telomeric complex
In order to confirm the bioinformatics data and to prove the hypothesis, the genomic DNA was extracted from the human dermal fibroblast (HDF) cells and restricted with specific restriction enzymes namely; Rsa I and Hinf I for telomeric DNA coating experiments. Restricted genomic DNA has intact telomeric repetitive sequences (TTAGGG)n. After coating of the telomeric repetitive DNA, lamin A and trf2 specific antibodies were added into protein lysate and determined the lamin A as well as trf2 binding capability. The data was shown in the (Figure 1C), which clearly illustrates that lamin A binds strongly with the telomere repetitive DNA sequences (TTAGGG)n. Similarly, as a positive control trf2 shows high fluorescence intensity, which indicates that trf2 has strongly bound with telomere repetitive DNA. Interestingly, the fluorescence intensity of trf2 is higher than lamin A, which indicates that very short percentage of lamin A amino acid residues may interact with the telomere repetitive DNA sequences. Further to validate the data, we performed co-immunoprecipitation.