2.5 SDS PAGE and Immunoblotting
Immunoprecipitated protein sample was resolved in 10% SDS-PAGE,
transferred to the PVDF membrane and blocked with 4% BSA followed by
incubation with primary antibody (anti-lamin A antibody). The membrane
was washed with 1X TBST buffer and incubated with secondary antibody
conjugated with HRP. The membrane was developed with DAB substrate and
the results were documented using gel documentation system.