2.10 Nuclear morphological study
Wild type and HGPS lamin A mutated cells were cultured on a coverslip in 6 well plate. Cells were incubated with primary anti-lamin A antibody followed by secondary antibody, anti-rabbit IgG Alexa Fluor 647. The slides were counter stained with DAPI, mounted using the mounting medium and observed under the EVOS epi-fluorescence microscope for nuclear morphological analysis.