3.1 Prediction of lamin A and telomeric DNA interaction
Human lamin A protein sequence was retrieved from the protein data bank and used for the prediction of telomeric repetitive DNA sequences (TTAGGG)n binding capability. Briefly, the bioinformatics tool namely DP bind was used for this study. The default prediction parameters were used for the analysis as suggested by the algorithm. The prediction of lamin A and telomeric DNA binding interaction was shown in (Figure 1A). The prediction clearly shows that amino acid residues of lamin A from 607 to 646 have high proximity towards telomeric repetitive DNA sequences. In addition, the algorithm scoring indicates that the tail region of lamin A was predicted as high level of interaction with telomeric DNA sequences. The known telomere repeat binding protein trf2 was selected as a positive control for DP bind analysis. Similarly, trf2 protein sequence was retrieved from protein data bank and used for the prediction of telomeric DNA binding capability. The result was shown in the (Figure 1B), which confirms that the DP bind algorithm shows accurate result, that telomeric DNA binding domain of trf2 show high proximity towards telomeric DNA sequences. Based on the preliminary data, further experiments were performed to prove the following hypothesis; 1. Interaction of lamin A with trf2 and 2. Interaction of lamin A with telomere repetitive DNA sequences (TTAGGG)n.