2.10 Nuclear morphological study
Wild type and HGPS lamin A mutated cells were cultured on a coverslip in
6 well plate. Cells were incubated with primary anti-lamin A antibody
followed by secondary antibody, anti-rabbit IgG Alexa Fluor 647. The
slides were counter stained with DAPI, mounted using the mounting medium
and observed under the EVOS epi-fluorescence microscope for nuclear
morphological analysis.