2.5 SDS PAGE and Immunoblotting
Immunoprecipitated protein sample was resolved in 10% SDS-PAGE, transferred to the PVDF membrane and blocked with 4% BSA followed by incubation with primary antibody (anti-lamin A antibody). The membrane was washed with 1X TBST buffer and incubated with secondary antibody conjugated with HRP. The membrane was developed with DAB substrate and the results were documented using gel documentation system.