2.9 Lamin A and trf2 interaction by Immunostaining
Wild and mutant transfected cells were grown on a coverslip in 6 well plates. Cells were fixed with ice-cold methanol followed by permeabilization with Triton X-100 and blocked with 1% BSA (Bovine Serum Albumin). After blocking, cells were incubated with primary antibodies (anti-lamin A and anti TRF2). Coverslip was washed with 1X PBS and incubated with fluorophore conjugated secondary antibodies, counterstained with DAPI. Coverslips were mounted and observed under EVOS epi-fluorescence microscope.