3.5 Nuclear morphometric analysis of HGPS mutant and wild type
lamin A
In order to confirm that, loss of 39 amino acid residues at C terminal
end of mature lamin A disrupts the structural integrity of the nucleus;
we performed wildtype and HGPS mutant lamin A transfection experiments.
Data for the mutant and wildtype lamin A transfection was shown in the
(Figure 2). The data clearly implies that, the structural integrity of
the nucleus was highly affected in HGPS mutant and noted as irregular
and lobulated nuclei. Whereas, wild type lamin A transfected fibroblast
cells show circular nuclei. This data confirms that, loss of 39 amino
acid residues at C terminal end of mutant lamin A causes the disruption
of nuclear structural integrity.