2.7 Vector Design and transfection
Wild type GFP lamin A construct was gifted from Professor Kaushik
Sengupta, Saha Institute of nuclear physics. Mutant lamin A (HG∆50)
construct was gifted from Prof. Junko Oshima from University of
Washington, USA. HDF cells were transfected with mutant HG∆50 and wild
type lamin A vectors through lipid-mediated gene transfer (Lipofactomin
3000, catalog: L3000008). The transfected cells were cultured at T-flask
in DMEM medium supplemented with 10% FBS under 5% CO2 at 37°C
separately, and used for further experiments, accordingly.