Immune cell type Biomarkers Assay References
Basophils Decrease in basophil activation post allergen-specific OIT (CD63, CD203, histamine release) BAT, iBAT 254-256
B cells
Induction of Breg cells on AIT, Serological readouts: food, and allergen-specific IgE, IgG4 Epitope-specific IgE and IgG4 Flow cytometry, ImmunoCAP, Eptiope mapping
170,234,243,257
T cells
Functional changes in tetramer positive/CFSElo/CD69+ CD40L+ allergen-specific CD4+ T cells, Tracking Th2A cells Decrease in T-cell derived type 2 cytokines Epigenetic changes in T cell subsets (Tregs) Flow and mass cytometry, Luminex/ELISA, pyrosequencing
258-263
Dendritic cells
Changes in costimulatory potential (CD80, CD86)
Flow cytometry, DC-T cell co-culture
253,264