Fig. 5 continued
Fig. 5. Pollution-induced trained immunity is epigenetically mediated by enhancing H3K27ac in circulating monocytes.Human circulating monocytes freshly enriched from a healthy individual were trained with the vehicle (negative control) or SRM1648a pollutant for 24h followed by resting for 6 days. ChIP Seq was utilized to study H3K27ac. Intersect plot consensus peaks call between pollutant versus vehicle-trained samples were determined. The specific, overlapping, and total number of the peaks per training condition were summarized in a Venn diagram (A). Peaks annotation stacked barplot was generated by calculating the proportion of peaks assigned to genomic features by HOMER (45 ) (B). Pileup heatmap of the H3K27ac mark specific to pollutant in comparison with medium at enhancers and TSSs. Rows are genomic regions from -3 to +3 kb around the center of the peaks; the signal intensity was determined in windows of 300 bp. (C). Genome browser screen shots of H3K27ac ChIP Seq landscape at IL1β, IL6, CXCL8, CCL3, AHR, IRF8, TCF3 , and SETD1A loci as representative inflammatory, transcription factor, and epigenetic signatures associated with pollution training. Super-enhancer regions of each gene are highlighted with dashed line boxes. (D). The KEGG enrichment analysis of the closest genes assigned to the pollutant-specific peaks was performed using MSigDB Hallmark 2020 (E).