Fig.
5 continued
Fig. 5. Pollution-induced trained immunity is
epigenetically mediated by enhancing H3K27ac in circulating monocytes.Human circulating monocytes freshly enriched from a healthy individual
were trained with the vehicle (negative control) or SRM1648a pollutant
for 24h followed by resting for 6 days. ChIP Seq was utilized to study
H3K27ac. Intersect plot consensus peaks call between pollutant versus
vehicle-trained samples were determined. The specific, overlapping, and
total number of the peaks per training condition were summarized in a
Venn diagram (A). Peaks annotation stacked barplot was generated by
calculating the proportion of peaks assigned to genomic features by
HOMER (45 ) (B). Pileup heatmap of the H3K27ac mark specific to
pollutant in comparison with medium at enhancers and TSSs. Rows are
genomic regions from -3 to +3 kb around the center of the peaks; the
signal intensity was determined in windows of 300 bp. (C). Genome
browser screen shots of H3K27ac ChIP Seq landscape at IL1β, IL6,
CXCL8, CCL3, AHR, IRF8, TCF3 , and SETD1A loci as representative
inflammatory, transcription factor, and epigenetic signatures associated
with pollution training. Super-enhancer regions of each gene are
highlighted with dashed line boxes. (D). The KEGG enrichment analysis of
the closest genes assigned to the pollutant-specific peaks was performed
using MSigDB Hallmark 2020 (E).