Transcriptome sequencing
SARS-coV-2 affects the cardiovascular system to a great extent and to
understand the effects of thalidomide and its derivatives, human
umbilical vein endothelial cells (HUVEC) were subjected to 20µM
thalidomide or 20µM lenalidomide or 20µM pomalidomide or vehicle control
treatment for 8h. RNA was isolated using TRIzol® method and whole
transcriptome sequencing was performed using Illumina HiSeq 2500
platform. The data can be accessed at Gene Expression Omnibus (GEO) with
the Accession ID: GSE118979. The sequence reads were aligned with
reference genome of Homo sapiens using TopHat2 (v2.0.8) and the
followed by transcript compilation and gene identification was done
using Cufflinks (v2.2.0) . The DEG were identified using Cuffdiff
program (v2.2.0)