Phenotypical analysis by flow cytometry
After the incubation period, specific proliferations were assessed by flow cytometry in a FACS Canto II cytometer (BD Biosciences Milpitas, USA) analysing the CFSEdim expression in CD3+, CD4+, CD8+and NK cells and in different subpopulations: CD3+CD4+CXCR3+IFNγ+(CD4+Th1); CD3+CD4+CRTH2+IL-4+(CD4+Th2)35; NK cells including the subtypes, CD3-CD56+Perforin+(NKPerf) and CD3-CD56+IFNγ+(NKIFN-γ).11 Data were analysed by FlowJo software (BD Biosciences Milpitas, USA) following different gate strategies (Figure S1B). Results were expressed as Proliferation Index (PI) calculated as (%CFSEdim stimulated lymphocytes+moDCs-%CFSEdim unstimulated lymphocytes+moDCs)/ %CFSEdimlymphocytes.35