2.2 Full-length genome sequencing
In order to sequence the full length of the SVV genome, we divided the full sequence into seven segments; primers were designed according to CH-01-2015 (Accession No. KT321458.1) (Table 1). The 5’-UTR and the 3’-poly(A) tail of SVV were sequenced by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR) (Sangon, China). All PCR products were purified with a Gel Extraction Kit and cloned into the pEASY-Blunt Simple vector (Transgene, China) in accordance with the manufacturer’s instructions. The resulting recombinant plasmid was then sequenced (Comate, China).