2.2 Full-length genome sequencing
In order to sequence the full length of the SVV genome, we divided the
full sequence into seven segments; primers were designed according to
CH-01-2015 (Accession No. KT321458.1) (Table 1). The 5’-UTR and the
3’-poly(A) tail of SVV were sequenced by rapid amplification of cDNA
ends (RACE) polymerase chain reaction (PCR) (Sangon, China). All PCR
products were purified with a Gel Extraction Kit and cloned into the
pEASY-Blunt Simple vector (Transgene, China) in accordance with the
manufacturer’s instructions. The resulting recombinant plasmid was then
sequenced (Comate, China).