2.3 Alignment, phylogenetic analysis, and the epidemiology of SVV in China
In total, we downloaded 99 complete SVV genomes from GenBank (Table 2) that were collected between 2002 and 2019 from seven different countries. Next, alignment and phylogenetic analysis was used to analyze the genomes of the isolated SVV; these analyses were based on the complete sequences of SVV and VP1. Analysis involved the P-distance-based neighbour-joining method in MEGA 7.0 software (Tamura et al., 2011). As VP1 is the main structural protein of SVV and may contain multiple antigen epitopes, we paid particular attention to the possibility of mutation in the amino acids of SVV. MegAlign software (DNASTAR.Lasergene.v7.1) was used to analyze mutations of the amino acids in VP1 of CH-GX-01-2019 and compare these with other isolates. The similarity of the CH-GX-01-2019 genome with other isolates was analyzed by MegAlign software (DNASTAR.Lasergene.v7.1).
In total, 53 SVV isolates in China were collected from GenBank (Table 2). We then analyzed the major genotypes of the strains involved. Then, we generated a genetic tree for the evolution of SVV evolution and then investigated the geographical distribution of the virus.