Immunofluorescence staining of CSF1R in LCH lesions
Six biopsy samples from LCH patients at diagnosis were collected for
immunofluorescence (IF) analysis. IF was performed as described.
Briefly, tissue sections were deparaffinized, dehydrated, incubated with
H2O2 for 20 minutes, subjected to
high-pressure repair for 2 minutes, blocked with BSA for 30 minutes, and
then incubated with anti-CSF1R and anti-BRAF -V600E antibodies at
4 °C overnight. The slices were incubated with the secondary antibody
and evaluated via fluorescence microscopy.