Immunofluorescence staining of CSF1R in LCH lesions
Six biopsy samples from LCH patients at diagnosis were collected for immunofluorescence (IF) analysis. IF was performed as described. Briefly, tissue sections were deparaffinized, dehydrated, incubated with H2O2 for 20 minutes, subjected to high-pressure repair for 2 minutes, blocked with BSA for 30 minutes, and then incubated with anti-CSF1R and anti-BRAF -V600E antibodies at 4 °C overnight. The slices were incubated with the secondary antibody and evaluated via fluorescence microscopy.