Genotyping for TLR9.rs5743836 (-1237T→C) and TLR9.rs352140 (G2848A) SNPs
PCR for TLR9.rs5743836 (-1237T→C) was carried out in a total reaction volume of 25 µl with 20 pmol of each of the forward and reverse primers as described [17]. On the other hand, PCR for TLR9.rs352140 (G2848A) was carried out in a total reaction volume of 25 µl with 20 pmol of each of the forward and reverse primers as described [16].