HCV-genotyping and genotyping for TLR3.rs3775290 (c.1377C/T) SNP
PCR-RFLP was used for HCV genotyping after reverse transcription as
described [15].
PCR-RFLP
was also performed to identify TLR3.rs3775290 (c.1377C/T) gene SNP. PCR
was carried out in a total reaction volume of 25 µl with 20 pmol of each
of the forward and reverse primers as described [16].