IL-10 producing cTfreg cells were decreased in MS patients
Being the most common anti-inflammatory cytokine, we analyzed and
compared the secretion of IL-10 between HC and MS patients. In PBMCs,
IL-10 secreting cells were significantly lower in MS patients compared
to HC (0.77±0.14% vs. 2.15±0.38%, p=0.004) (Fig. 6a,d). Next, we
investigated the production of IL-10 by follicular and non-follicular
cTreg cells, followed by comparative observation between HC and MS
patients. The results showed that compared to non-follicular cTreg
cells, higher percentage of cTfreg cells secreted IL-10 in both HC
(19.19±2.71% vs. 4.41±0.87%, p=0.0001) and MS patients (11.85±1.24%
vs. 2.39±0.23%, p<0.0001), respectively (Fig. 6b,c,e).
Similar to the trend of decreased frequency of cTfreg cells in MS, IL-10
producing cTfreg cells were significantly reduced in MS patients
compared to HC (11.85±1.24% vs. 19.19±2.71%, p=0.02) (Fig. 6b,c,e).
The percentage of IL-10 producing non-follicular cTreg cells were also
reduced in MS patients compared to HC (Fig. 6c,e).