eDNA metabarcoding
The 12S library generated 22.46 million raw sequence reads, with 13.51
million reads passing bioinformatic filters. After quality filtering and
removal of chimeric sequences, each reservoir sample contained 63,593
target reads (i.e. fish) on average. Zebra mbuna was the only fish
species detected in PCR positive controls. In negative controls
(field/filtration blanks, evaporation blanks, extraction blanks, and PCR
negative controls), no fish species were detected.