eDNA metabarcoding
The 12S library generated 22.46 million raw sequence reads, with 13.51 million reads passing bioinformatic filters. After quality filtering and removal of chimeric sequences, each reservoir sample contained 63,593 target reads (i.e. fish) on average. Zebra mbuna was the only fish species detected in PCR positive controls. In negative controls (field/filtration blanks, evaporation blanks, extraction blanks, and PCR negative controls), no fish species were detected.