2.2 | Extraction of CPV genome
Fecal swabs were rinsed with 1ml sterile phosphate-buffered saline
(PBS), and centrifuged at 12,000 × g and 4°C for 10 min, and the
supernatants were stored at −80℃. The small intestinal tissue
homogenates were clarified by centrifuging at 12,000×g and 4°C for 10
min to separate the supernatant. Then, 200 μL of supernatant from each
sample was filtered through a 0.22
μm
filter (Millipore, USA). The viral DNA was extracted from the
supernatant of the treated samples by an AxyPrepTMviral DNA miniprep kit (Axygen Scientific, Tewksbury, MA, USA) according
to the manufacturer’s instructions.