3.1 | PCR detection and genotype analysis
The PCR results showed that all 44 samples from domestic dogs were
positive for CPV-2. A 1775-bp fragment covering the full-length of VP2
was amplified and sequenced from all samples. All of the VP2 genes were
typed according to the key amino acid residues at positions 87, 101,
103, 297, 300, 305, 426 and 555. Detailed information from the 44
samples is provided in Table 1. Among the 44 CPV-positive samples, 31
CPVs were classified as CPV-2c (70.4%), 8 as new CPV-2a, 4 as new
CPV-2b (9.1%), and 1 as CPV-2.