Sample processing
Pig samples were separated, and serum analysed for the presence of antibodies against JEV using indirect IgG ELISA (H Dhanze et al., 2019) and indirect IgM ELISA (H. Dhanze, Bhilegaonkar, et al., 2020). The diagnostic sensitivity and specificity of the indirect IgG ELISA was 91% and 97%, respectively, while the diagnostic sensitivity and specificity of the indirect IgM ELISA was 95.34% and 98.6%, respectively. The IgM ELISA for sero-diagnosis of JEV was developed and validated in 2017 and therefore, samples prior to September 2017 were screened only for the presence of IgG antibodies against JEV.