RPL26 gene annotation and verifying differential expression in
CRSwNP by PCR
Through functional analysis of the core network as well as ribosome
signaling pathway anchoring genes and NCBI database annotation we found
that all the 15 key genes in the ribosome signaling pathway were
included in the 16 core network interacting genes. There were 3
up-regulated genes and 13 down-regulated genes(Fig.3C). Through NCBI
database and GenCards website notes, we found that RPL26 gene played an
important role in this process. To verify whether RPL26 was
differentially expressed in CRSwNP, we cultured nasal polyp tissue cells
in vitro again. Normal nasal mucosa group (Control), nasal polyp group
(CON) and dexamethasone intervention group (DTSG) were cultured for 3
days. All the cells were collected for quantitative PCR analysis. The
results indicated that the RPL26 gene expression in nasal polyp tissues
was lower normal nasal mucosa, but after dexamethasone intervention, the
RPL26 gene expression level was significantly increased(Fig.4).