Transcriptome sequencing
The Agilent used in this experiment was Mouse lncRNA Microrray V3 (4*180K, Design ID: 084388) . Data analysis were conducted by OE Biotechnology Co., Ltd., (Shanghai, China). the NanoDrop ND-2000 (Thermo Scientific) was used to quantifed Total RNA,and the RNA integrity was assessed by Agilent Bioanalyzer 2100 (Agilent Technologies). The sample labeling, microarray hybridization and washing were performed based on the standard protocols. Total RNA were transcribed to double strand cDNA, then synthesized into cRNA and labeled with Cyanine-3-CTP. The labeled cRNAs were hybridized onto the microarray. After washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies). Feature Extraction software (version10.7.1.1, Agilent Technologies) was used to analyze array images to get raw data. Genespring (version 14.8, Agilent Technologies) were employed to finish the basic analysis with the raw data.