Cell apoptosis was analyzed by flow cytometry
Part of the samples were digested by trypsin without EDTA in each group
and then collected by centrifugation. After repeated washing with PBS,
cell resuspension solution was added for resuscitation, then apoptotic
solution (5ul FITC staining solution and 10ul PI staining solution) was
added, and the cells were left at 37℃ darkroom for 10min. After adding
cell resuspension solution again, the samples were measured by computer
to count the apoptosis rate.