CRSwNP tissue and Cell culture
This study was approved by the Ethics Committee of the Tenth People’s
Hospital of Tongji University and the written consent of all specimen
providers were obtained. 8 patients with CRSwNP were randomly selected
from June 2019 to September 2019 in the otolaryngology department of the
Tenth People’s Hospital of Tongji University. The diagnosis was based on
European Position Paper on Rhinosinusitis and Nasal Polyps。
Tissue culture in vitro: The nasal polyp tissue sample obtained during
the operation was repeatedly washed with PBS solution and cut into
tissue pieces of about 5mm 3 on the clean bench. Then the pieces were
disinfected with iodine and digested, then filtered by a sieve,
centrifuged, resuspended and placed in a 6-well plate for culture and
passage. Randomly divided into two groups: dexamethasone intervention
treatment group (DSTG) group and normal CRSwNP group (CON), 4 for each
group.