Cell lines and animals
SK-N-SH cells, obtained from Shanghai Cell Bank of Chinese Academy of
Medical Sciences, were maintained in DMEM (Gibco) with 10% fetal calf
serum (Gibco), and cultivated at 37°C in a 5% CO2humidified incubator. 6 week female BALB/c mice, each weighing 18 to 20
g (SCXK Hebei 2008-1-003), were purchased from the Experimental Animal
Center of Hebei Province (SPF grade, SYXK Hebei 2008-0026) and housed in
a specific pathogen-free facility. Spirit, diet and defecation of mice
were regularly observed and recorded. All experiments were conducted in
accordance with the principles and procedures outlined in the Guideline
of Institutional Experimental Animal Review Committee.