Extraction, identification and cultivation of
CD3+T cells
CD3+T cells were extracted from mice spleen
lymphocytes suspension by Mouse CD3+Tcell Magnetic
Bead. Magnetic-activated cell sorting (MACS) can be repeated for several
times and about 3×106 T lymphocytes can be extracted
from each mouse spleen. The purified lymphocytes were collected and
identified purity of CD3+T cells. Concentration of
cells was adjusted to 5×106/ml and cultivated 2 days
in medium containing rmIL-2 (20 ng/ml). Then, cell morphology was
observed under the phase contrast microscope and cell vitality was
tested by Trypan blue.