Preparation of SK-N-SH Ag-specific CTL and cytokine release test
After CD3+T cells were cultivated for 2 days, DCs loaded tumor antigen were added according to the proportion of DC:T = 1:20. At the same time, rmIL-2 (20 ng/ml) was mixed and cultivated for 3 or 4 days. Thereafter, morphology of T cells was observed under inverted microscope. After T cells had been activated for 72 hours, the supernatants in each group were retained and concentrations of IL-2 and IFN-γ were calculated respectively according to the absorbance value and the standard curve measured by enzyme standard instrument. IL-2 and IFN-γ in the supernatant between the different groups were compared.