Preparation of SK-N-SH Ag-specific CTL and cytokine
release test
After CD3+T cells were cultivated for 2 days, DCs
loaded tumor antigen were added according to the proportion of DC:T =
1:20. At the same time, rmIL-2 (20 ng/ml) was mixed and cultivated for 3
or 4 days. Thereafter, morphology of T cells was observed under inverted
microscope. After T cells had been activated for 72 hours, the
supernatants in each group were retained and concentrations of IL-2 and
IFN-γ were calculated respectively according to the absorbance value and
the standard curve measured by enzyme standard instrument. IL-2 and
IFN-γ in the supernatant between the different groups were compared.