MDSC inhibiting proliferation of CTL
Under fluorescence microscopy, the number of CTLs was same in the two groups before cultivation (Fig. 2A). However, after having been cultivated for 4 days, cells proliferated obviously but staining intensity weakened in CTL group. On the contrary, in CTL+MDSC group, cell number scarcely increased and staining intensity still remain strong (Fige. 2B).
Analysis of flow cytometry showed consistent results. In CTL+MDSC group, CTLs with strong fluorescence (87.6%) were much more than CTLs with weak fluorescence. However, strong fluorescence CTLs (44.1%) were less than weak fluorescence CTLs in CTL group (Fig. 2C).