Viruses, cells and antibodies
The recombinant baculoviruses were grown in Spodoptera frugiperda
clone 9 (Sf9) suspension cells, which were maintained in SFM II 900
medium (Thermo Fisher Scientific, Waltham, MA, USA) at 27°C and 120 rpm
on a horizontal shaker. The ZIKV ZKC2 2016 strain was cultivated in
C6/36 cells (MEM with 10% FBS) at 27°C.
The anti-flavivirus E monoclonal antibody (mAb) D1-4G2-4-15 was
purchased from Millipore (Billerica, MA, USA). The rabbit anti-ZIKV E
polyclonal antibody was purchased from GeneTex (Alton PkwyIrvine, CA,
USA). Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG,
horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG and
HRP-conjugated goat anti-mouse IgG were purchased from Abcam (Cambridge,
MA, USA). HRP-conjugated goat anti-mouse IgG (H+L) were purchased from
BioWorld (St. Louis, MN, USA). HRP-conjugated goat anti-mouse IgG1 or
IgG2a were purchased from BioWorld SouthernBiotech (Birmingham, AL,
USA). Purified ZIKV-E protein from Escherichia coli was purchased
from MyBioSource (San Diego, CA, USA). Fluorophore-labeled
monoclonal antibodies for flow cytometry were purchased from BD
Biosciences (Franklin, CA, USA).