Flow cytometry assays of staining of cell-surface molecule
staining
A total of 5×105 splenocytes were cultured and
stimulated with the purified ZIKV-E protein (10 μg/ml) for 72 h. Then,
the cells were collected and stained with anti-CD4 (FITC), anti-CD8
(PE), anti-CD19 (APC), anti-CD69 (PE/Cy7), anti-CD44 (APC), and
anti-CD62L (PerCP-Cy5.5) antibodies. Data were analyzed using a
FACSCalibur flow cytometer (BD Biosciences, Franklin, CA, USA).