Western blot (WB) analysis
The recombinant baculovirus was inoculated into Sf9 cells at an MOI of 0.5, and the mixture was harvested on the 4th day. The precipitate and supernatant were separated by centrifugation, and the precipitate was sonicated to obtain sonicated supernatant. Samples were denatured in loading buffer at 100°C for 5 min and separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Then, the separated proteins were transferred onto nitrocellulose (NC) membranes (GE Healthcare, Little Chalfont, Buckinghamshire, UK) for immunoblot analysis. A polyclonal rabbit anti-ZIKV-E antibody (1:700) was used as the primary antibody, and HRP-conjugated goat anti-rabbit IgG (1:5000) was used as the secondary antibody. SuperSignal West Dual Chemiluminescent substrate (Pierce, Rockford, IL, USA) was added as the chromogen, and bands were imaged using a Tanon-5200 Chemiluminescent Imaging System (Tanon, Shanghai, China).