Cytokine and chemokine response
CCL2, CCL3, IL-8, OSM and LIF were expressed at high levels at baseline (Table 1). IL-13, IL-8, LIF, HGF, and CCL2 (Figure 2, a-c, e, g) and membrane bound CSF-1 and FL3L (Figure 3, h, i) were only upregulated through high affinity IgE activation at all times investigated. OSM, CCL3 and CCL4 were released by stimulation through both low- and high affinity IgE activation at 3 hours, but at higher levels for high affinity IgE (Figure 2, d, h, i). Production through high affinity IgE activation of CCL3 and CCL4 had ceased at 24 hours. CCL7/MCP3 (Figure 2, j) was significantly upregulated through low affinity IgE activation.
Cytoplasmic markers in the supernatant
CASP-8 (Figure 2, k) was significantly upregulated with low affinity IgE and differentially regulated with low- and high affinity IgE activation. SIRT2 (Figure 2, l) was significantly upregulated with low affinity IgE but not with high affinity IgE activation. STAMBP (Figure 2, m) was upregulated through high affinity IgE activation only.