IgE antibodies for mast cell sensitization
After 6 weeks of culture each cell line was divided in two aliquots that were sensitized with 80 kU/L recombinant human IgE (rIgE) containing 7% each of non-overlapping Der p 2-specific IgE clones in combination with 86% of a non-allergen-specific IgE antibody (8); clones H10:H12 (kD 284 nM) and P4C (kD 291 nM) for low affinity stimulation and clones H12 (kD 1.12 nM) and P4E (kD 1.4 nM) for high affinity stimulation. The difference in affinity of IgE between the conditions was thus approximately 200-fold.