2.3 Statistical analysis
A mid-point probit analysis (written in R programming language for statistical computing and graphics) was used to determine the amount of heparin (μg/ml) required to reduce SARS-CoV-2 viral plaques by 50% (ND50) compared with the virus only control (n=10). Analysis was conducted in R (Project, 2019) and the script was based on a source script from Johnson et al 2013 (Johnson, Dahlgren et al., 2013).