3.5. Virus isolation and replication in cell cultures
Virus isolation results showed that after inoculating homogeneous tissue supernatant into LMH cells, the second-generation cell culture produced CPE in LMH cells within 24 hours after inoculation (Fig. 3A). And nearly 90% of the cells showed obvious CPE in 72 hours after inoculation (Fig. 3B). The observed CPE was consistent with the classic CPE of ARV, the characteristic giant or bloom‐like CPE and the cells fall off in clusters. Based on this, it can be judged that we isolated N-DRV from duck embryos in group. And then, we confirmed the N-DRV positive using RT-PCR amplification. This re-isolated strain was named N-DRV-SD19