3.5. Virus isolation and replication in cell cultures
Virus isolation results showed that after inoculating homogeneous tissue
supernatant into LMH cells, the second-generation cell culture produced
CPE in LMH cells within 24 hours after inoculation (Fig. 3A). And nearly
90% of the cells showed obvious CPE in 72 hours after inoculation (Fig.
3B). The observed CPE was consistent with the classic CPE of ARV, the
characteristic giant or bloomâlike CPE and the cells fall off in
clusters. Based on this, it can be judged that we isolated N-DRV from
duck embryos in group. And then, we confirmed the N-DRV positive using
RT-PCR amplification. This re-isolated strain was named N-DRV-SD19