Figure 2. Duck embryos died during the incubation and the
pathological changes in Embryo and ducklings.
(A) The eggs of the experimental group vary in size; (B) The number of
inconsistent shape eggs and sand-shelled eggs increased; (C)
Unfertilized egg in infected group; (D) Some duck embryos had difficulty
in breaking shells at the later stage of hatching; (E) Severe bleeding
of embryo body; (F) Dead duck embryo with hemorrhage in liver, (1)
Infected group duck embryo and (2) control duck embryo; (G) Some
ducklings were depressed; (H) Spleen of duckling presents with severe
splenic necrosis.
Figure 3. CPE in N-DRV-SD19 infected LMH cells. (A) The second
generation of LMH cells showed CPEs 24 hr after infection; (B) The
second generation of LMH cells showed CPEs 72 hr after infection; (C)
Non-infected LMH cell 24 hr after test; (D) Non-infected LMH cell 72 hr
after test.
Figure 4. Phylogenetic tree constructed based on nucleotide
sequences of S1 genome segment by Orthoreovirus species.