2.3 Infectivity and titer determination of pseudotyped EBOV
For infectivity detection, various target cells from different species (Huh-7, HEK293T, A549, C6/36, Vero, BHK, BSR) were infected with pseudotyped EBOV serially diluted in DMEM without FBS. Then DMEM containing 10% FBS was added to replace DMEM without FBS at 4 h post-infection, and relative luciferase units (RLUs) were measured 48 h later. After normalized by OD value to negative control, positive result was recognized as more than 3.0 under a definitive dilution.