2.2 Recombinant plasmid construction and generation of EBOV pseudotyped virus
The complete genome of EBOV is shown in Fig. 1a, and the full-length G gene of EBOV (GenBank accession no. KJ660346.2) was synthesized by Sangon Biotech Biotechnology Co., Ltd. (Shanghai, China) and cloned into the pcDNA4.0 vector. The recombinant plasmid was confirmed by enzyme digestion analysis and by DNA sequencing, and the details of the recombinant plasmid are shown in Fig. 1b. The pseudotyped EBOVs was produced by the transfection of HEK293T cells with a two-plasmid system, which consists of the GP-encoding plasmid (pcDNA4.0-G) and luciferase-expressing plasmid (pNL4-3.luc.RE).