3.2 Incorporation of EBOV GP into pseudotyped virus particles
To identify whether EBOV GP was expressed in the pseudotyped EBOV
system, western blotting was performed. As shown in Fig. 1d, both EBOV
GP and HIV-1 p24 protein were identified in the pseudotyped EBOV. TEM
was performed to analyze the morphology of pseudotyped EBOV particles.
As shown in Fig. 1e, pseudotyped EBOV particles with diameters of
approximately 100-120 nm were observed in pseudotyped virus-containing
supernatants, outer envelop protein, EBOV GP, were observed on the
surface of pseudotyped EBOV, which revealed efficient packaging and
secretion of pseudotyped EBOV. The result of IEM observation was shown
in Fig. 1f, multiple gold particles were found to bind to EBOV GP on the
surface of pseudotyped virus. Altogether, the data suggests that EBOV GP
was effectively incorporated onto the surface of HIV-1 backbone, and
pseudotyped EBOV were packaged and rescued.