Sequencing
Whole-genome sequencing was performed using an Ion Torrent Personal
Genome Machine (PGM) System (Life Technologies), following the
manufacturer’s specifications. Sequencing libraries were constructed
using the Ion Plus Fragment Library (Life Technologies) by physical
fragmentation of genome segments with the Bioruptor® Sonication System
to generate fragments of approximately 200 base pairs (bp). The DNA
fragments were linked to Ion‐compatible adapters and amplified using the
Ion PGM™Hi‐Q™ OT2 Kit (Life Technologies) on an Ion OneTouch™ 2 System.
The sequencing reaction was carried out using an Ion 314 Chip v2 and the
Ion PGM™ Hi‐Q™ Sequencing Kit (Life Technologies). Reads were aligned
with a Q‐score ≥ 20 and assembled with the AssemblerSPAdes v 5.4.0
program. The average genomic coverage depth of the Ion Torrent PGM was
up to 300‐fold.