Sequencing
Whole-genome sequencing was performed using an Ion Torrent Personal Genome Machine (PGM) System (Life Technologies), following the manufacturer’s specifications. Sequencing libraries were constructed using the Ion Plus Fragment Library (Life Technologies) by physical fragmentation of genome segments with the Bioruptor® Sonication System to generate fragments of approximately 200 base pairs (bp). The DNA fragments were linked to Ion‐compatible adapters and amplified using the Ion PGM™Hi‐Q™ OT2 Kit (Life Technologies) on an Ion OneTouch™ 2 System. The sequencing reaction was carried out using an Ion 314 Chip v2 and the Ion PGM™ Hi‐Q™ Sequencing Kit (Life Technologies). Reads were aligned with a Q‐score ≥ 20 and assembled with the AssemblerSPAdes v 5.4.0 program. The average genomic coverage depth of the Ion Torrent PGM was up to 300‐fold.