Epithelial-derived miR-29s in exosomes
BEAS-2B cells were stimulated with a combination of 100 ng/ml IL-4 and TNF-α for 48 h. Cell culture supernatants were collected, and the exosomal fractions were extracted. A, The expression levels of miR-29a and miR-29b in the exosomal fractions were measured by qPCR. The data are shown as the mean ± SD of triplicate samples. Statistical analysis was performed by Student’s t -test. *P < .05. B, The expression of CD81, a marker of exosomes, was analyzed by Western blot analysis.